ROCHE Trypsin Inhibitorfrom soybean

Not available outside of the UK & Ireland.

Application

Trypsin Inhibitor has been used: in the buffer for hepatocyte isolationto inhibit trypsin in Corneal epithelial cellsto inhibit papain activity while treating hippocampal neural stem cells (NSCs)

Trypsin Inhibitor from soybean is used to terminate tissue disaggregation and for subcultivation procedures.

Biochem/physiol Actions

Soybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. The product is sensitive to heat, alkaline pH, and protein-precipitating compounds.

Soybean trypsin inhibitor (STI) inhibits myc proto-oncogene protein and is anticarcinogenic. It favors anti-aggregation of platelets and may be a potential protease inhibiting drug. STI inhibits renin and angiotensin.

General description

Soybean trypsin inhibitor (STI) comprises 181 amino acids. It is a monomeric protein corresponding to a molecular weight of 20 kDa and has two disulfide bridges. It belongs to serpins − serine protease inhibitors family.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Working concentration: 10 to 100 µg/mlWorking solution: Recommended solvent is distilled water.Stock solution: 1 mg/mlStorage conditions (working solution): -15 to -25 °CThe enzyme is stable for 6 months, frozen in aliquots.

Reconstitution

Sensitive to heat, alkaline pH, and protein-precipitating compounds.

Specifications

Inhibiting activity: approximately 70 trypsin inhibitor units/mg lyophilizate at +25°C with benzoyl-L-arginine ethylester as a substrate (approximately 190 trypsin inhibitor units/mg at +25°C with Chromozym TRY as substrate)Isoelectric point: 4.5

Specificity

Soybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. It inhibits trypsin, factor Xa, plasmin, and plasma kallikrein activity in serum-free cell culture media. It does not inhibit metallo-, cysteine, aspartic proteases, or tissue kallikrein (serine proteases).

Storage and Stability

Store at 2 to 8 °C. (Store dry!)

Unit Definition

1 U = 270 S & T-units or 90 USP-units