Not available outside of the UK & Ireland.

Application

Chicken egg Trypsin Inhibitor is used for the termination of tissue disaggregation and for subcultivation procedures.

Trypsin Inhibitor has been used: as a component of lysis buffer to treat Tsc2fl/fl(control) andTsc2fl/flLyz2-Cre (knockout [KO]) bone marrow-derived macrophages (BMDMs) before immunoblotting to inactivate trypsin post detachment of the stem cells in ventricular zone cell cultureto neutralize trypsin post dissociation and trypsinization of brain tissuestermination of tissue disaggregation and for subcultivation procedures

General description

Egg white trypsin inhibitor is an inhibitor for trypsin, plasmin and plasma kallikrein activity in serum-free cell-culture media. It does not inhibit metallo-, cysteine, and aspartic proteases or tissue kallikrein (serine protease).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Working concentration: 0Working solution: Solvent is recommended in distilled water.Stock solution: 1 mg/mlStorage conditions (working solution): -15 to -25 °CStable for at least 6 months in distilled water or 1 mM HCI when stored frozen in aliquots. Avoid repeated freeze-thaw cycles.

Reconstitution

Both are soluble in water. Recommended stock solution: 1 mg/ml. Store frozen in aliquots at -15 to -25 °C. Stable for at least 6 months.

Storage and Stability

Store at 2 to 8 °C. (Store dry!)