Not available outside of the UK & Ireland.

Application

Polyadenylic acid (Poly(A)) to inhibit Exo1′s exonuclease activity. It has also been used as a carrier to resuspend lyophilized gBlocks.

Poly(A) has been used for droplet digital PCR (ddPCR) assay.

Biochem/physiol Actions

Polyadenylic acid (Poly(A)) tails present at 3′ end are produced in the cell nucleus. It contains ~250 nucleotides in mammalian cells.Poly(A) regulates mRNA decay and the initiation of translation. Cytoplasmic poly(A) extension modulates translation.

General description

Poly(A) is used as a carrier for quantitative precipitation of DNA and RNA.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

Lyophilizate, potassium salt

Preparation Note

Working concentration: 0.5 mg/mlA concentration of 0.5 mg/ml is recommended.Working solution: 0.5 mg/ml is recommended.

Quality

Typical analysis:2.3µmol/mg Poly(A) (from absorbance) in relation to one mononucleotide unit. Chromatographically homogeneous.Absorbance determination A₂₅₀/A₂₆₀, A₂₈₀/A₂₆₀, A₂₉₀/A₂₆₀

Sequence

Chain Length 2.100 to 10.000 nucleotides

Unit Definition

1 A₂₆₀ unit corresponds to 40 µg ssRNA.