Not available outside of the UK & Ireland.

Application

Nuclease S7 has been used in ribonuclease selection process for ribosome profiling.

Removal of endogenous RNA from in vitro translation systemsRNA sequencing experiments

Biochem/physiol Actions

Nuclease S7 is an endonuclease that mainly cleaves single-stranded substrates. It also cleaves double-stranded DNA or RNA. It cleaves the 5′-phosphodiester bonds of RNA and DNA to yield 3′-mononucleotides and oligonucleotides. Activity of the enzyme is dependent on Ca²⁺ and can be completely inactivated by the addition of EDTA. Nuclease S7 cleavage is highly specific to AT rich region than GC on the DNA.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage and Stability

Store unopened reagent at 2-8 °C; when diluted to 1 mg/mL, store at -15 to -25 °C.

Unit Definition

One unit releases a sufficient amount of acid-soluble oligonucleotides to produce an increase of 1.0 at A₂₆₀.