Not available outside of the UK & Ireland.

General description

D-galactose:NAD⁺ 1-oxidoreductase

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

Suspension in 3.2 M ammonium sulfate solution, 1 mM EDTA, pH approximately 6

Quality

Contaminants: ﹤0.01% ADH, ﹤0.01% ?-galactosidase, ﹤0.1% NADH oxidase, ﹤0.5% LDH

Sequence

Galactose dehydrogenase from Pseudomonas fluorescens (MW 64,000 D) is a dimer with subunits of equal size (subunit MW = 32,000 D).

Specificity

Galactose dehydrogenase will oxidize D-galactose [K_m = 0.7 mM (30 °C, pH 9.1); relative rate = 1.00], L-arabinose (relative rate = 0.47), D-fucose (relative rate = 1.05), 2-deoxy-D-galactose (relative rate = 0.27), 2-amino-2-deoxy-D-galactose [galactosamine], (relative rate = 0.02; pH 8.6).Galactose dehydrogenase does not oxidize L-galactose, D-arabinose, D-glucose, L-fucose, D-ribose, D-xylose, D-glucuronic acid, D-galacturonic acid or D-galactose-6-phosphate. The enzyme will use either NAD (K_m = 0.24 mM; relative rate = 1.0) or NADP (K_m = 2.3 mM; relative rate = 0.32).

Unit Definition

One unit galactose dehydrogenase will oxidize 1 mol of β-D-galactose in one minute at +25 °C and pH 8.6.The QC assay produces 1 mol of NADH per mol of galactose oxidized.