HighPrep™ PCR is a paramagnetic beads-based post PCR clean-up reagent designed for efficient purification of amplicons and tight size-specific selection of DNA fragments. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic bead particles. Highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications, such as library construction, next generation sequencing (NGS), Sanger sequencing, cloning, restriction digestions, adapter ligation, microarrays, and so on. This protocol can be used for manual procedures as well as in automation.

Applications: post-PCR and post-enzymatic reaction clean-up used for/during:

• NGS library preparation
• DNA size selection for NGS
• Microarrays
• PCR
• Restriction enzyme digestions, adapter ligations
• Cloning

Benefits

• Rapid and reliable post-PCR and DNA clean-up reagent
• Achieve uniform and consistent DNA fragments
• High recovery of amplicons >100 bp
• Uniform fragments size distribution
• Adaptable to high throughput liquid handling workstations