Not available outside of the UK & Ireland.

Analysis Note

Absorption: Absorption maximum in the visible light is not determined.

Application

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

General description

BCIP serves as substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually detected.

Legal Information

BCIP is a registered trademark of Sigma-Aldrich Co. LLC

Other Notes

For life science research only. Not for use in diagnostic procedures.

Quality

Performance test: incubation with AP

Substrates

Histochemical substrate for alkaline phosphatase.