Not available outside of the UK & Ireland.

Application

Yeast Nitrogen Base Without Amino Acids and Ammonium Sulfate is used in classifying yeasts based on carbon and nitrogen requirements. It contains all essential nutrients required for the growth of yeasts except amino acids, nitrogen and carbohydrate. The addition of nitrogen and carbon sources is required.

Components

IngredientsBiotin, 2 µg/LCalcium pantothenate, 400 µg/LFolic acid, 2 µg/LNiacin, 400 µg/Lp-Aminobenzoic acid, 200 µg/LPyridoxine HCl, 400 µg/LRiboflavin, 200 µg/LThiamine HCl, 400 µg/LInositol, 2 mg/LBoric acid, 500 µg/LCopper sulfate, 40 µg/LPotassium iodide, 100 µg/LFerric chloride, 200 µg/LManganese sulfate, 400 µg/LSodium molybdate, 200 µg/LZinc sulfate, 400 µg/LPotassium phosphate monobasic, 1 g/LMagnesium sulfate, 0.5 g/LSodium chloride, 0.1 g/LCalcium chloride, 0.1 g/L

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

General description

Yeast Nitrogen Base Without Amino Acids and Ammonium Sulfate is recommended to use for classifying yeasts based on their carbon and nitrogen requirements. The medium contains all essential nutrients required for the growth of yeasts except amino acids, histidine, methionine and tryptophan, and also ammonium sulphate. The addition of nitrogen and carbon sources is required as described in the Manual of Clinical Microbiology

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

100 g in poly bottle

1 kg in poly bottle

Preparation Note

Prepare a 10× stock solution by suspending 1.7 g of yeast nitrogen base without amino acids and ammonium sulfate in 100 ml of cold distilled water. Include desired nitrogen and carbon sources. Warm if necessary to solubilize and sterilize by filtration. Store 10× stock solution at 2-8°C. To use, dilute 1:10 with sterile distilled water under aspetic conditions.