Not available outside of the UK & Ireland.

Application

Used for isolation and enumeration of wild yeasts in pitching yeasts.

Components

Ingredients (g/L)Dextrose, 44.50Monopotassium phosphate, 1.78Magnesium sulphate, 0.89Calcium chloride, 0.178Sodium chloride, 0.089Adenine, 0.00178DL-Methionine, 0.000891L-Histidine, 0.000891DL-Tryptophane, 0.000891Boric acid, 0.0000089Zinc sulphate, 0.0000356Ammonium molybdate, 0.0000178Manganese sulphate, 0.0000356Ferrous sulphate, 0.0002225L-Lysine, 1.00Inositol, 0.02Calcium pantothenate, 0.002Aneurine, 0.0004Pyridoxine, 0.0004p-Aminobenzoic acid, 0.0002Nicotinic acid, 0.0004Riboflavin, 0.0002Biotin, 0.000002Folic acid, 0.000001Agar, 17.80

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Preparation Note

Suspend 6.6 g of Lysine Medium in 100 ml of distilled water containing 0.84 ml of potassium lactate solution ~60% (Cat. No. 60389). Boil to dissolve the medium completely. Cool to 50 °C. Adjust the pH to 5.0 with a 10% solution of Lactic Acid (Cat. No. 69778) and pour into sterile petri plates.NoteThe degree of contamination is expressed as the number of wild yeast cells per million cells of the original inoculum. The number of cells in the inoculum is important as a small number (100-1000) grow to a limited extent while 10′000 brewing yeast cells provide a direct measure of contaminant wild yeasts.