Not available outside of the UK & Ireland.

Application

Recommended for the microbiological assay of folic acid using Streptococcus faecium as the test organism.

Components

Components (g/L)Casein Acid Hydrolysate, Vitamin Free 12.00Dextrose 40.00Sodium Citrate 20.00L-Cystine 0.20DL-Tryptophan 0.20Adenine Sulfate 0.02Guanine Hydrochloride 0.02Uracil 0.02Thiamine Hydrochloride 0.002Pyridoxine Hydrochloride 0.004Riboflavin (Vitamin B₂) 0.002Nacin 0.002p-Aminobenzoic Acid (PABA) 0.0002Biotin 0.0000008Calcium Pantothenate 0.0004Dipotassium Phosphate 1.00Monopotassium Phosphate 1.00Magnesium Sulfate 0.40Sodium Chloride 0.02Ferrous Sulphate 0.02Manganese Sulphate 0.02

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Preparation Note

Suspend 7.5 g of Folic Acid Assay Medium in 100 ml of distilled water. Boil to dissolve the medium completely. Mix well to distribute the slight precipitate evenly. For assay, dispense 5 ml of medium per assay tube, and add 5 ml of distilled water to each tube. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 10 minutes. Cool immediately.