Not available outside of the UK & Ireland.

Application

Recommended for microbiological assay of Vitamin B₁₂.

Components

Ingredients (g/L)Casein acid hydrolysate, 15.00Dextrose, 40.00Asparagine, 0.20Sodium acetate, 20.00Ascorbic acid, 4.00L-Cystine, 0.40DL-Tryptophan, 0.40Adenine sulfate, 0.02Guanine hydrochloride, 0.02Uracil, 0.02Xanthine, 0.02Riboflavin (Vitamin B₂), 0.001Thiamine hydrochloride, 0.001Biotin, 0.00001Niacin, 0.002p-Amino benzoic acid, 0.002Calcium pantothenate, 0.001Pyridoxine hydrochloride, 0.004Pyridoxal hydrochloride, 0.004Pyridoxamine hydrochloride, 0.0008Folic acid, 0.0002Monopotassium phosphate, 1.00Dipotassium phosphate, 1.00Magnesium sulfate, 0.40Sodium chloride, 0.02Ferrous sulfate, 0.02Manganese sulfate, 0.02Polysorbate 80, 2.00

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Preparation Note

Suspend 8.5 g of Vitamin B12 Assay Medium in 100 ml of distilled water. Boil to dissolve the medium completely. Mix well to distribute the slight precipitate evenly. Dispense 5 ml of medium into tubes containing increasing concentrations of cyanocobalamin (Cat. No. 43107) standard or the unknown. The total volume of 10 ml per tube is adjusted by adding distilled water. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool the medium.