Not available outside of the UK & Ireland.

Application

Recommended for use in classification of yeasts on the basis of their ability to assimilate nitrogen compounds.

Components

Ingredients (g/L)Dextrose, 10.00L-Histidine hydrochloride, 0.001DL-Methionine, 0.002DL-Tryptophan, 0.002Biotin, 0.000002Calcium pantothenate, 0.0004Folic acid, 0.000002Inositol, 0.002Niacin, 0.0004p-Amino benzoic acid, 0.0002Pyridoxine hydrochloride, 0.0004Riboflavin (Vitamin B₂), 0.0002Thiamine hydrochloride, 0.0004Boric acid, 0.0005Coppers sulfate, 0.00004Potassium iodide, 0.0001Ferric chloride, 0.0002Manganese sulfate, 0.0004Sodium molybdate, 0.0002Zinc sulfate, 0.0004Monopotassium phosphate, 1.00Magnesium sulfate, 0.50Sodium chloride, 0.10Calcium chloride, 0.10

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Preparation Note

A. Nitrogen Assimilation testPrepare the broth base at 10 x concentration by dissolving 11.7 grams of Yeast Carbon Base in 100 mL of distilled water. Add the appropriate nitrogen source. Warm if necessary to dissolve the medium completely. Sterilize by filtration.B. Detection of yeasts, other than Saccharomyces cerevisaeDissolve 2.35 grams of Yeast Carbon Base in 100 mL of distilled water.C. Detection of wild yeasts in beer and other brewing componentsAdd 0.33 g of ammonium sulphate (Catalog No. A2939) and 4 g of bacteriological agar (Catalog No. A5306) to base B. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.