Not available outside of the UK & Ireland.

Application

PhotoClick Cholesterol has been used: in lysosome-associated membrane protein 1 (LAMP1) crosslinking studiesas an analog of cholesterol in binding and competition assay of sterol binding proteins from yeastas a conjugate with the ectodomain of lysosomal integral membrane protein-2 (LIMP-2)

Biochem/physiol Actions

PhotoClick Cholesterol serves as a probe for tracking cholesterol-binding proteins. The copper-catalyzed azide-alkyne cycloaddition is the basis for its probe functionality. The alkyne group is essential for the click chemistry property. Its binding to partners is mediated by photocrosslinking.

General description

PhotoClick Cholesterol is a synthetic analog of cholesterol and is photoreactive. It comprises the alkyne group.

PhotoClick Cholesterol is a clickable, photoreactive sterol probe for use in combination with quantitative mass spectrometry to globally map cholesterol-protein interactions directly in living cells. In addition to the UV-activatable cross-linking diazirine group that reacts with proteins, the sterol contains an alkyne group for incorporation of reporter and affinity tags through click-chemistry.

Packaging

2 mL Amber Serum Vial with Stopper and Crimp Cap (700147P-100ug)

2 mL Amber Serum Vial with Stopper and Crimp Cap (700147P-250ug)