Not available outside of the UK & Ireland.

Application

Acetyl-CoA carboxylase is responsible for synthesis of Malonyl-CoA which is an inhibitor of fatty acid oxidation in skeletal muscle mitochondria. The enzyme may be used to study the effect on production of malonyl-CoA as well as fatty acid oxidation during exercise. The enzyme also may be used for ACC regulation study in anti-obesity and anti-type 2 diabetes therapeutics.

Biochem/physiol Actions

Acetyl-CoA Carboxylase (ACC) regulates the metabolism of fatty acids. This enzyme catalzes the formation of Malonyl CoA through the irreversible carboxylation of acetyl CoA. There are two main isoforms of Acetyl-CoA carboxylase expressed in mammals, Acetyl-CoA carboxylase 1 (ACACA) and Acetyl-CoA carboxylase 2 (ACACB). ACACA has broad tissue distribution but is enriched in tissues critical for fatty acid sythesis such as adipose tissue. ACACB is enriched in tissues such as skeletal muscle and heart that are critical for fatty acid oxidation. The Acetyl-CoA Carboxylase enzymes are activated by citrate, glutamate, and dicarboxylic acids and negatively regulated by long and short chain fatty acyl CoAs. Because of thier roles in fatty acid metabolism and oxidation, ACACA and ACACB are therapeutic targets for treating obesity and metabolic syndrome disorders.

Physical form

Buffered aqueous solution containing Tris-HCl, pH 8.0, NaCl, glycerol, and DTT. May also contain one or more of the following: EDTA, KCl, imidazole, TWEEN-20.

Physical properties

C-terminal HIS-tagged 277 kDa full length protein

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit will cause the carboxylation of 1 picomole of acetyl-CoA per minute at pH 7.4 at 30 deg C.