Ready-To-Go T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the 5′-phosphoryl group and the 3′-hydroxyl group of two double-stranded DNA fragments. Ready-To-Go T4 DNA Ligase includes self-circularization of linear DNA prior to transformation, ligation of insert to vector, and ligation of synthetic linkers to blunt-ended DNA.
- Covalently-linking DNA fragments takes only 30 minutes
- Single-dose, ambient temperature stable reaction mixes
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