AlkPhos Direct Labeling and Detection Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase. CDP-Star or ECF detection can be used.

DNA or RNA probes are labeled during a 30min reaction and resulting probes can be used without purification. Hybridization stringency can be controlled by temperature as well as salt concentration. Detection is rapid, taking only 30 min from completion of hybridization to application of detection reagent.

Each system includes the following reagents, sufficient for 25 or 50 labeling reactions and detection of 2500cm² or 5000cm² membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, and blocking agent.