Autofluor LS315

Code: nat1446 D2-176

Autofluor represents the first water-soluble scintillation phosphor to be developed and applied directly for use as an autoradiographic image intensifier. Autofluor rapidly penetrates acrylamide ge...


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$256.65 1L

Autofluor represents the first water-soluble scintillation phosphor to be developed and applied directly for use as an autoradiographic image intensifier. Autofluor rapidly penetrates acrylamide gel systems and maximises energy transfer from labelled compound to phosphor. Autofluor contains no dimethylsulfoxide or acidic aromatic solvents. Therefore, the hazards of use related to these materials are eliminated. The band distortion that is associated with using nonaqueous enhancers is also eliminated.

  • High-resolution autoradiographic image intensifier
  • Rapid enhancement of low energy beta-emitters
  • For polyacrylamide gels, paper chromatography, and TLC plates
  • Water-based, odorless, contains no DMSO
  • Higher degree of resolution and band discrimination with Autofluor vs PPO-DMSO
  • Autofluor procedure is the shortest and easiest procedure yet developed for enhancement and visualisation of beta-emitter
  • In an independent test comparing eight different fluorographic methods for the detection of 35S-labeled proteins in polyacrylamide gels, Autofluor was the most effective, with Autofluor, the dpm/mm2 required to half-saturate the x-ray film was 1/8 that required by autoradiography alone
  • Autofluor has a shelf life of at least one year stored at room temperature, out of direct sunlight, keep from freezing, at temperatures less than 20°C precipitation of water soluble phosphors may occur, warming to approximately 30°C will redissolve the phosphors

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