BIOshell A400 Protein C4, 3.4 mum HPLC Column, 3.4 mum particle size, L x I.D. 15 cm x 4.6 mm

Code: 66829-u D2-231

Not available outside of the UK & Ireland.

General description

BIOshell™ 400 Å Protein C4 columns contain 3.4 µm advanced Fused-Core particles with 400 Å pores that are specifically engineered to...


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Not available outside of the UK & Ireland.

General description

BIOshell™ 400 Å Protein C4 columns contain 3.4 µm advanced Fused-Core particles with 400 Å pores that are specifically engineered to provide efficient separation of larger proteins. These columns provide excellent separations on very hydrophobic proteins such as those found in the cell membrane. The C4 ligand combined with an exhaustively endcapped surface ensures optimum protein recovery of larger or very hydrophobic biomolecules. The 3.4 µm Fused-Core particles allow a shorter analysis time yet similar back pressure to fully porous 1.7 µm, 300 Å fully porous C4 particles. These columns can be operated long term at pressures up to 9000 psi (600 bar), temperatures up to 90 °C, and within the pH range of 2 to 9.

Legal Information

BIOshell is a trademark of Sigma-Aldrich Co. LLC

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agencysuitable for USP L26
descriptionShell thickness (0.2 µm), Solid Core (3.0 µm)
extent of labeling0.4% carbon loading
featureendcapped
L × I.D.15 cm × 4.6 mm
manufacturer/tradenameBIOshell™
materialstainless steel hardware
matrix active groupC4 (butyl) phase
matrixsuperficially porous particle, spherical silica particle platform
operating pH range2-9
packaging1 ea of
parameter90 °C max. temp., 600 bar max. pressure
particle size3.4 µm
pore size400 Å
product lineBIOshell™
Quality Level100
separation techniquereversed phase
surface area15 m2/g
surface coverage4.2 µmol/m2
technique(s)UHPLC-MS: suitable, HPLC: suitable, LC/MS: suitable, UHPLC: suitable
This product has met the following criteria: