Discovery BIO Wide Pore C18 HPLC Column, 5 mum particle size, L x I.D. 15 cm x 4.6 mm

Code: 568222-u D2-231

Not available outside of the UK & Ireland.

General description

Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RP-HPLC column must be...


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$694.55 EACH
$833.46 inc. VAT

Not available outside of the UK & Ireland.

General description

Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RP-HPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information. Discovery BIO Wide Pore C18 gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry we have incorporated into the Discovery BIO Wide Pore line improve resolution by increasing efficiency and reducing the peak tailing. An added benefit to this is the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.

Legal Information

Discovery is a registered trademark of Sigma-Aldrich Co. LLC

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agencysuitable for USP L1
extent of labeling9.2% Carbon loading
featureendcapped
L × I.D.15 cm × 4.6 mm
manufacturer/tradenameDiscovery®
materialstainless steel column
matrix active groupC18 (octadecyl) phase
matrixfully porous particle, silica particle platform
operating pH2-8
packaging1 ea of
parameter400 bar pressure (5801 psi), 0-70 °C temperature
particle size5 µm
pore size300 Å
product lineDiscovery®
Quality Level100
separation techniquereversed phase
surface area100 m2/g
surface coverage3.6 µmol/m2
technique(s)HPLC: suitable, LC/MS: suitable
This product has met the following criteria: