Not available outside of the UK & Ireland.

Analysis Note

Enzymatic activity assays are performed in Tris-HCl buffer (100 mM, pH 7.5) containing Neu-5-Ac (10 mM) at 37 °C for 15 min and analyzed using capillary electrophoresis with UV detection at 200 nm.

Application

Sialic acid aldolase can be used to synthesize unnatural sugars of C(6) to C(10) for the design of antagonists and inhibitors of glycoenzymes.

General description

Sialic acid aldolases, or N-acetylneuraminate lyases, catalyze the reversible aldol cleavage of N-acetylneuraminic acid to form pyruvate and N-acetyl-D-mannosamine. In nature, N-acetylneuraminate lyase mainly occurs in pathogens.

Physical form

Lyophilized powder containing Tris-HCl and NaCl

Unit Definition

One unit will catalyze the formation of 1.0 µmol Neu-5-Ac from Man-N-Ac and pyruvate per minute at 37°C at pH 8.0.