Not available outside of the UK & Ireland.

Analysis Note

Protein determined by TCA Biuret.

Application

3-Phosphoglyceric Phosphokinase generates ATP by catalyzing the transfer of a phosphate group from 1,3-diphosphoglycerate to ADP. 3-Phosphoglycerate Phosphokinase is used to study glycolysis and gluconeogenesis. It has also been used to study low molecular weight GTP-binding proteins and mechanisms of inhibition of glyceraldehyde-3-phosphate dehydrogenase.

The enzyme has been used in the assay of glyceraldehyde-3-phosphate dehydrogenase.

Biochem/physiol Actions

3-Phosphoglyceric Phosphokinase catalyzes the reversible transfer of a phosphate group from 1,3-diphosphoglycerate to ADP to generate ATP and 3-phosphoglycerate. 3-Phosphoglycerate Phosphokinase activity is essential for glycolysis and gluconeogenesis.

Physical form

Crystalline suspension in 3.0 M (NH₄)₂SO₄ and 0.04 M tetrasodium pyrophosphate solution, pH 8.0

Unit Definition

One unit will convert 1.0 µmole of 1,3-diphosphoglycerate to 3-phosphoglycerate per min at pH 6.9 at 25 °C.