Not available outside of the UK & Ireland.

Application

Pyruvate Oxidase from Aerococcus sp. has been used in the amplex Red-based fluorescence assay for pyruvate. It is suitable for use in the preparation of biosensor for determination of thiamine (vitamin B1), and as a component in the constant part of the multi-enzyme biocatalytic cascade for the determination of biomarkers for traumatic brain injury (TBI) and soft tissue injury (STI).

Pyruvate Oxidase (PoxB) converts pyruvate directly to acetate and CO₂. It is used to study pyruvate metabolism. It is used to study aerobic metabolism of bacterium, such as Lactobacillus plantarumand Streptococcus pneumoniae.

Biochem/physiol Actions

Pyruvate Oxidase oxidises pyruvate to form acetate, hydrogen peroxide and carbon dioxide. Pyruvate oxidase requires flavin adenine dinucleotide (FAD), thiamine pyrophosphate (TPP) and magnesium as cofactors for its catalytic activity. Thiamine activates pyruvate oxidase activity.

Pyruvate Oxidase consists of four subunits with identical molecular weights. PoxB reacts with certain aldehydes and phosphate can be replaced by arsenate. Oxygen as well as several artificial compounds can function as electron acceptors. Pyruvate Oxidase is activated by phospholipids as well as monomeric and micellar amphiphiles.

Physical form

Lyophilized powder containing buffer salt and sugar

Unit Definition

One unit will produce 1.0 µmole of H₂O₂ per min during the conversion of pyruvate and phosphate to acetylphosphate and CO₂ at pH 6.7 at 37 °C.