Not available outside of the UK & Ireland.

Application

Liberates mannose from a variety of synthetic and natural α-mannosides.

α-Mannosidase can be used to liberate mannose from a variety of synthetic and natural α-mannosides. It has also been used in a study to investigate the causes of neurodegeneration in mucolipidosis II ′knock-in′ mice.

α-Mannosidase from Canavalia ensiformis (Jack bean) has been used:for the digestion of neutral glycans.to remove O-glycans from recombinant β-glucosidasefor the inhibition of renal fibroblasts proliferation

Biochem/physiol Actions

α−Mannosidase has been shown to inhibit the proliferation of B-lymphocytes.

α-Mannosidase is an acid hydrolase which is located in plant vacuoles and is thought to be involved with the turnover of N-linked glycoproteins.

α-Mannosidase cleaves near α1−2-, α1−3- and α1−6-linked mannose residues.

General description

α−Mannosidase from Canavalia ensiformis is a tretamer composed of two subunits that each contain two components at 44 and 66 kDa.

Lysosomal α-mannosidases are class II mannosidases that belong to glycoside hydrolase family 38 and play an important role in the degradation of asparagine-linked carbohydrates of glycoproteins.

α-Mannosidase is an exo-glycosidase and an inc metalloenzyme.

Physical form

Suspension in 3.0 M (NH₄)₂SO₄ and 0.1 mM zinc acetate, pH 7.5

Unit Definition

One unit will hydrolyze 1.0 µmole of p-nitrophenyl α-D-mannoside to p-nitrophenol and D-mannose per min at pH 4.5 at 25°C.