Not available outside of the UK & Ireland.

Application

Lipoprotein Lipase from bovine milk has been used:as a supplement to test its effect on DiI (1,1′-dioctadecyl-3,3,3′-tetramethyl-indocarbocyanine perchlorate)- very-low-density lipoprotein (VLDL) uptake in breast cancer MDA-MB-231 cells to treat human brain microvascular endothelial cells (HBMECs) for the lipolysis of triglyceride-rich lipoproteins (TGRL)to test its effect on gene expression in normal human astrocytes

Biochem/physiol Actions

Lipoprotein Lipase (LPL) from bovine milk contributes to maximal lipolytic activity. It associates with casein micelle. LPL regulates triglyceride utilization and displays positional specificity. Lipases, in general, catalyzes the lipolysis of triglycerides especially at the fatty acid in sn-1 andsn-3 positions of the triglyceride.

General description

Lipoprotein Lipase (LPL) from bovine milk is a glycoprotein. It exists as a homodimer and comprises twoN-linked oligosaccharides. It is heat-labile.

Packaging

5000 units in glass bottle

Physical form

Suspension in 3.8 M ammonium sulfate, 0.02 M Tris HCl, pH 8.0

Preparation Note

Affinity purified

Unit Definition

One unit will release 1.0 nmole of p-nitrophenol per min at pH 7.2 at 37 °C using p-nitrophenyl butyrate as substrate.