Not available outside of the UK & Ireland.

Analysis Note

Protein determined by biuret.

Application

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Lipase from porcine pancreas has been used:in lipase inhibition assay with plant extracts in in vitro digestion gelatinized starch/fiber mixtureswith simulated intestinal fluidto mimic the digestive tract condition of by L. fermentum PC1 in fermented oats

Biochem/physiol Actions

Lipase from porcine pancreas (PPL) catalyzes the hydrolysis of fats (lipids). It plays an essential role in the digestion, transport, and processing of dietary lipids (e.g. triglycerides, fats, oils) in most living organisms. PPL is cost-effective and is useful in biotransformation reactions. It displays high selectivity, and catalytic activity. PPL is also used in the detergent formulation for removing oil stains from clothes.

Lipases catalyze the hydrolysis of esters in aqueous solutions and the synthesis of esters in non-aqueous solutions. They also produce hydroxyl and carboxylic groups through the hydrolysis of ester linkages in poly(ethylene terephthalate).Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

General description

Lipase from porcine pancreas (PPL) or porcine pancreatic lipase is a small globular protein with N-terminal α/β type fold. It comprises catalytic triad Ser, Asp, and His residues and a C-terminal β-sandwichdomain. PPL belongs to a subclass of the carboxylesterases family.

Packaging

1000000 units in poly bottle

100000 units in glass bottle

Unit Definition

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.7 at 37 °C using olive oil.