Not available outside of the UK & Ireland.

Application

Glucose-6-phosphate dehydrogenase is used to test ketose reductase activity in developing maize endosperm.

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

General description

Glucose 6-phosphate dehydrogenase (G-6-P-DH) is a key regulatory enzyme in the first step of the pentose phosphate pathway. G-6-P-DH is a glycoprotein with a molecular mass of 128 kDa (gel filtration).

Physical form

Suspension in 3.2 M (NH₄)₂SO₄ containing 50 mM Tris and 1 mM MgCl₂, pH 7.5

Unit Definition

One unit will oxidize 1.0 µmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.