Not available outside of the UK & Ireland.

Application

β-glucuronidase was used in enzymic hydrolysis of tissue homogenates for liquid chromatography-electrospray ion trap mass spectrometry (LC/MSⁿ) analysis, to study the structures of degradation products of baicalin.

Biochem/physiol Actions

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

Other Notes

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Packaging

1000000 units in poly bottle

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 µg of phenolphthalein from phenolphthalein glucuronide per hr at 37°C at the pH 5.0 (30 min assay).

One unit of sulfatase will hydrolyze 1.0 µmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.