Not available outside of the UK & Ireland.

Analysis Note

Protein determined by biuret.

Activity using maltose as substrate at pH 6.0 at 25 deg C is ~2X greater than that obtained using p-nitrophenyl-α-D-glucoside as substrate at pH 6.8 at 37 °C.

Application

α-glucosidase is potential enzyme for the biosynthesis of complex carbohydrates.

Biochem/physiol Actions

α-glucosidase hydrolyzes carbohydrates by acting on 1,4-α linkages. Inhibition of α-glucosidase is a prominent target in the management of non-insulin-dependent diabetes mellitus.

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch hydrolysis research. For more information see the article in biofiles.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Packaging

250 units in glass bottle

Physical form

Lyophilized powder containing potassium phosphate buffer salt

Unit Definition

One unit will liberate 1.0 µmole of D-glucose from p-nitrophenyl α-D-glucoside per min at pH 6.8 at 37 °C.