Not available outside of the UK & Ireland.

Application

Glycerol Dehydrogenase (GDH) from Cellulomonas sp. has been used in the determination of glycerol by sequential injection analysis (SIA) method. It has also been used in the preparation of pyrroloquinoline quinone (PQQ) based biosensor for the determination of glycerol.

This enzyme is useful for enzymatic determination of glycerol and of triglyceride when coupled with lipoprotein lipase in clinical analysis. Formation of NADH from the reaction of glycerol and NAD⁺ was catalyzed by the enzyme glycerol dehydrogenase.

Biochem/physiol Actions

Glycerol dehydrogenase catalyzes the conversion of glycerol to glycerone.

General description

Glycerol Dehydrogenase belongs to iron-containing alcohol dehydrogenase family. It is made up of eight identical subunits which are about 42,000 Da each. The C-terminal has histidine and the N-terminal has serine amino acid.

Packaging

250, 1000 units in poly bottle

Physical form

Lyophilized powder containing bovine serum albumin

Physical properties

Isoelectric point : 4.4 ± 0.1Michaelis constants : 1.1 x 10¯²M (Glycerol), 8.9 x 10¯⁵M (NAD⁺⁾Structure : 10 subunits (42,000) per mol of enzymeInhibitors : p-Chloromercuribenzoate, o-phenanthroline, monoiodoacetate, heavy metalions (Co++, Ni⁺⁺, Cu⁺⁺, Zn⁺⁺, Cd⁺⁺)Optimum pH : 10.0 – 10.5 Optimum temperature : 50°C pH Stability : pH 7.5 – 10.5 (25°C, 20hr) Thermal stability : below 55°C (pH 7.5, 15min) Substrate specificity : This enzyme has the highest specificity for glycerol and 1,2-propanediol, and also oxidizes glycerol-A-monochlorohydrin, ethyleneglycol and 2,3-butanediol. The oxidative reaction is stimulated by K⁺, NH⁺4 and Rb⁺.

Unit Definition

One unit will oxidize 1.0 µmole of glycerol to dihydroxyacetone per min at 25 °C at pH 10.0.