Not available outside of the UK & Ireland.

Application

Esterase from rabbit liver has been used in a study to investigate a toxic effect of carbamate insecticides. Esterase from rabbit liver has also been used in a study to investigate the effect of simvastatin on expression and activity of a lipoprotein-associated phospholipase A(2).

Esterase from rabbit liver has been used:in comparative biophysical studies with bovine bile-salt-activated lipase in the assays with vinyl acetate, triacylglycerols, p-nitrophenyl esters and serine enzyme inhibitorsin the catalysis and kinetic profiling studies of peptidiccompoundsin in vitro stability assays of prodrug compounds (BMS-292655 and BMS300043)in the hydrolysis of oleuropein aglycone (Ole-AG)

The enzyme from Sigma has been used to study the effect of divalent metal ions on the activity of esterase.

Biochem/physiol Actions

Esterase enzyme catalyzes hydrolysis of carboxylic ester to carboxylate and alcohol in the presence of water. Rabbit liver carboxylesterase catalyzes enantioselective hydrolysis of cineol derivatives.

Esterase enzyme has broad substrate specificity. Esterase from rabbit liver has been used to investigate the toxic effect of carbamate insecticides. Esterase from rabbit liver has also been used for the hydrolysis of simvastatin.

General description

Esterase belongs to the C-family of carboxylesterases. It is a glycoprotein, mainly present in the endoplasmic reticulum of hepatocytes.

Packaging

500, 1000 units in poly bottle

Physical form

Lyophilized powder containing Tris buffer salts

Unit Definition

One unit will hydrolyze 1.0 µmole of ethyl butyrate to butyric acid and ethanol per min at pH 8.0 at 25 °C.