Not available outside of the UK & Ireland.

Application

Creatinine Deiminase microbial has been used:to immobilize aminosilylated glass beads based biosensor for ammonia/ammonium and creatinine detection in urinein creating creatinine-sensing membrane for biophysical studiesto investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples

Creatinine deiminase has been used in a study to assess the application of a creatinine-sensitive biosensor for hemodialysis control. Creatinine deiminase has also been used in a study to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples.

Biochem/physiol Actions

Creatinine deiminase catalyzes the hydrolysis of creatinine to methylhydantoine and ammonia.

Packaging

250 units in poly bottle

Physical form

Lyophilized powder containing mannitol as stabilizer

Physical properties

Isoelectric point : 4.4Michaelis constant : 3.5 x 10‾³M (Creatinine)Structure : 6 subunits per mol of enzymeInhibitors : Ag⁺,Hg⁺⁺, o-phenanthroline,monoiodoacetateOptimum pH : 8.5 – 9.5 Optimum temperature : 65 – 75°C pH Stability : pH 7.0 – 11.0 (30°C, 20hr) Thermal stability : Below 65°C (pH 7.5, 1hr)

Unit Definition

One unit will hydrolyze 1.0 µmole of creatinine to N-methylhydantoin and NH₃ per min at pH 7.5 at 37 °C in a coupled system with L-glutamic dehydrogenase.