Not available outside of the UK & Ireland.

Application

Chitosanase from Sigma has been used for polarized light-stimulated, enzymatic hydrolysis of chitin and chitosan.

Biochem/physiol Actions

Chitosanase catalyzes the endohydrolysis of β-1,4-linkages between D-glucosamine (GlcN-GlcN) residues in chitosan. The enzyme from Streptomyces has been reported to also hydrolyze the GlcNAc-GlcN linkage in partially acetylated chitosan.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Physical form

Solution in 50% glycerol containing 50 mM sodium acetate

Preparation Note

Soluble in 10mL of 0.1 M Acetate buffer at pH 5.5

Unit Definition

One unit will liberate 1.0 µmole of reducing sugar (measured as D-glucosamine equivalents) from chitosan per minute at pH 5.5 at 37 °C.