Not available outside of the UK & Ireland.

Analysis Note

Protein determined by biuret.

Application

Adenosine Deaminase from bovine spleen has been used in the immobilization on biostrip for stability and catalytic studies.

Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays . Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease.

Biochem/physiol Actions

Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease.

General description

Molecular weight: 32.5-33 kDapI: 4.85

Physical form

Solution in 50% glycerol, 5 mM potassium phosphate, pH 6.0

Unit Definition

One unit will deaminate 1.0 µmole of adenosine to inosine per min at pH 7.5 at 25 °C.