Phosphatase, Alkaline from bovine intestinal mucosa, buffered aqueous glycerol solution, >=5,500 DEA units/mg protein

Code: a2356-50ku D2-231

Not available outside of the UK & Ireland.

Analysis Note

Package sizes are based on DEA units

Application

Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alk...


read more

Your Price
£817.00 EACH

Not available outside of the UK & Ireland.

Analysis Note

Package sizes are based on DEA units

Application

Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase..

High specific activity grade recommended for antibody and protein conjugation.

Biochem/physiol Actions

The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It is routinely used to dephosphorylate proteins and nucleic acids.

Physical form

Solution in 50% glycerol containing 5 mM Tris, 5 mM MgCl2 and 0.1 mM ZnCl2, pH 7.0

Physical properties

Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.2 The enzyme requires zinc, and magnesium or calcium divalent ions for activity.

Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.

Unit Definition

One DEA unit will hydrolyze 1 µmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. (One glycine unit is equivalent to ~3 DEA units)

concentration≥10 mg/mL
formbuffered aqueous glycerol solution
mol wtdimer ~160 kDa
Quality Level200
specific activity≥5,500 DEA units/mg protein
storage temp.2-8°C
Code
Description
Unit Size
List Price
Qty
Cas Number9001-78-9
This product has met the following criteria: