Not available outside of the UK & Ireland.

Application

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation . Product A2230 is a high activity apyrase.

At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (~10) and another with a low ratio (~1).Reaction: ATP → ADP+P_i → AMP+2P_i.

Biochem/physiol Actions

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis . Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

Packaging

Sold on the basis of ATPase units.

100 units in glass bottle

Physical form

Lyophilized powder containing potassium succinate buffer salts.

Preparation Note

Derived from red potato

Unit Definition

One unit will liberate 1.0 µmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.