Not available outside of the UK & Ireland.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

General description

Human USP14 (Ubiquitin-Specific Protease 14) or TGT (tRNA-guanine transglycosylase), amino acids 2-494 (end) with an N-terminal FLAG-tag, expressed in a baculovirus-infected Sf9 cell expression system. Ubiquitin-Specific Protease 14 is a deubiquitinating protein responsible for the removal of ubquitin tags attached to proteins by αNH-peptide bonds and the ε-NH-isopeptide bond.

USP14 has been shown to be phosphorylated by AKT which is required for protein stability and nuclear localization.

Physical form

Solution in 40 mM Tris-HCl, 110 mM NaCl, 2.2 mM KCl, 80 ng/µl Flag peptide, 3 mM DTT, and 20% Glycerol at pH 8.0.