Not available outside of the UK & Ireland.

Application

Sulfatase from Helix pomatia is often used to remove sulfate groups from carbohydrates.

General description

Sequential action of sulfatase from Helix pomatia and β-O-glucosidase from Caldicellulosiruptor saccharolyticus on glucosinolates allows synthesis of thiohydroximates. Sulfatase from Helix pomatia hydrolyzes β-naphthyl sulfate much faster than α-naphthyl sulfate.

Unit Definition

One unit will hydrolyze 1.0 µmole of p-nitrocatechol sulfate per hour at pH 5.0 at 37 °C (30 min assay).