Not available outside of the UK & Ireland.

Analysis Note

Protein determined by biuret.

Application

The enzyme has been used to create an oxygen scavenging system along with protocatechuate (PCA) and Trolox. The enzyme employs a nonheme iron center that catalyzes the conversion of PCA and molecular oxygen into β-carboxy-cis,cis-muconic acid, while the antioxidant Trolox suppresses slow blinking and photobleaching of cyanine dyes. It has been used in the preparation of imaging buffer along with DMB-BSA (dynein motility buffer-BSA), ATP and protocatechuate in single molecule motility assay.

Protocatechuate 3,4-Dioxygenase(PCD), from Pseudomonas sp., is used for the enzymatic determination of choline esterase when coupled with phydroxybenzoate hydroxylase. It is used to improve organic fluorophore-stability in single-molecule experiments and is used to study the metabolism of protocatechuate in Rhizobiaceae.

Biochem/physiol Actions

Protocatechuate 3,4-Dioxygenase catalyzes the degradation of 3,4-dihydroxybenzoate (protocatechuate) into β-carboxy-cis,cis-muconate.

General description

Protocatechuate 3,4-Dioxygenase belongs to the non-heme iron family of enzymes. The active site of the enzyme contains Fe³⁺.

Packaging

25 units in glass bottle

Physical form

Supplied as lyophilized powder.

Physical properties

Structure : Protein with nonheme ironInhibitors : Ag⁺, Hg^++, PCMBOptimum pH : 9.0 Optimum temperature : 60−65°C pH Stability : pH 7.0−9.0 (25°C, 72hr) Thermal stability : below 50°C (pH 6.0, 1hr)

Unit Definition

One unit will oxidize 1.0 µmole of protocatechuate to 3-carboxy-cis,cis-muconate per min at pH 7.5 at 37 °C.