Not available outside of the UK & Ireland.

Application

The product has been used for the measurement of pyrophosphate content of plant tissues. It has also been used as a rapid, routine check of pyrophosphate content by monitoring NADH oxidation in Solanum tuberosum tuber extracts.

Biochem/physiol Actions

The pyrophosphate detection kit couples the production of pyrophosphate to the consumption of NADH.

Components

Component ConcentrationImidazole × HCl, pH 7.4 45 mMCitrate 5 mMEDTA 0.10 mMMg²⁺, Mn²⁺, Co²⁺ 2 mM, 0.2 mM, 0.02 mMβ-NADH 0.8 mMF-6-P 12 mMBovine Serum Albumin 5 mg/mLSugar Stabilizer 5 mg/mLPPi-PFK 0.5 units/mLAldolase 7.5 units/mLGDH 5 units/mLTPI 50 units/mL

Packaging

A technical bulletin is included.

Preparation Note

Dissolve the contents of one vial of Pyrophosphate Reagent in 4.0 mL of water.

Principle

Two moles of NADH are oxidized to NAD⁺ per mole of pyrophosphate consumed. The reaction is monitored spectrophotometrically at 340 nm.The reagent can be used to detect 10-100 nmoles of pyrophosphate.

Quality

A coupled enzyme system containing fructose-6-phosphate kinase (pyrophosphate-dependent), aldolase, triosephosphate isomerase, and A-glycerophosphate dehydrogenase.