Not available outside of the UK & Ireland.

Application

Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Product P4630 is from bovine pancreas. Protease from bovine pancrease (type I) has been used for the extraction of hemicellulose.

Non-specific Protease Activity Assay Video

Biochem/physiol Actions

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate.

Packaging

1, 5, 10 g in poly bottle

250 mg in poly bottle

Quality

Crude

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 µmole (181 µg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.