Not available outside of the UK & Ireland.

Analysis Note

Protein determined by biuret.

Application

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. Alkaline phosphatase is also used to prevent DNA self ligation and for radiolabeling.

Biochem/physiol Actions

Alkaline phosphatase is a hydrolase enzyme that dephosphorylates molecules such as nucleotides, proteins and alkaloids.

Packaging

Package size based on DEA units

100 units in serum bottle

Physical form

Lyophilized fromTris-HCl buffer, with magnesium chloride and zinc chloride, pH 8.0

Unit Definition

One unit will hydrolyze 1.0 µmole of p-nitrophenyl phosphate per min at pH 9.8 at 37 °C.