Not available outside of the UK & Ireland.

Application

Phosphodiesterase I from Crotalus adamanteus venom has been used:for cleaving the 3′-5′ internucleotide bonds in the linear chains of the RNA oligomersas a model for the hydrolysis of prodrugs in phosphodiesterase assayas a component in the nucleotide cleavage buffer

Phosphodiesterase (PDE) is any enzyme that is used to breaks phosphodiester bonds. It is a membrane-bound glycoprotein that is used to catalyze the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is used in phosphodiesterase activation assays to hydrolyze AMP. Product P3134 is from crude, dried Crotalus adamanteus venom.

Biochem/physiol Actions

Phosphodiesterase I breaks phosphodiester bonds and catalyzes the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is released from eucaryotic plasma membranes by phosphatidylinositol-specific phospholipase C.