Not available outside of the UK & Ireland.

Application

Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Pectinase from Rhizopus sp has been used for enzymatic digestion of leaves.

Biochem/physiol Actions

Pectinases hydrolyses pectin, which is a component of the cell wall.They may attack methyl-esterified pectin or de-esterified pectin. It is a source of pectinase activity, also containing cellulase and hemicellulase activities.

General description

We are committed to bringing youGreenerAlternative Products, which adhere to one or more of The 12 Principles ofGreenerChemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.Pectinase, also known as pectinolytic enzyme, is predominantly found in bacteria, fungi and plants. Polygalacturonase is one of the highly abundant enzymes among all the pectinolytic enzymes. Pectinase has a wide range of industrial applications including, fruit juice extraction and its clarification. In addition, it is also used in vegetable oil extraction, tea and coffee fermentations, bleaching of paper, in poultry feed additives and in alcoholic beverages and food industries.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Unit Definition

One unit will liberate 1.0 µmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25 °C.