Not available outside of the UK & Ireland.

Analysis Note

Protein determined by biuret

Application

Pyruvate kinase from Bacillus stearothermophilus has been used in a study to assess evidence that the genes for phosphofructokinase and pyruvate kinase constitute an operon. It has also been used in a study to investigate the importance of the Lys221 active site for pyruvate kinase activity.

Biochem/physiol Actions

Pyruvate Kinase from Bacillus stearothermophilus is activated by AMP and ribose 5-phosphate.

Packaging

1000 units in glass bottle

Physical form

Lyophilized powder containing Tris buffer salts, pH 8.5

Unit Definition

One unit will convert 1.0 µmole of phospho(enol)pyruvate to pyruvate per min at pH 7.2 at 30 °C.