Not available outside of the UK & Ireland.

Application

Malic dehydrogenase has been used in a study to assess the comparative molluscicidal action of Ginko biloba extract on snail hosts. It has also been used in a study to investigate the effect of early feed restriction on metabolic programming and compensatory growth in broiler chickens.

Biochem/physiol Actions

Malic Dehydrogenase can be reversibly inactivated by treatment with pyridoxal 5′-phosphate at 25 °C.

Eukaryotic cells contain two different isozymes of malate dehydrogenase: mitochondrial (m-MDH) and soluble or cytoplasmic (s-MDH). This product consists of the mitochondrial form.The enzyme catalyzes the following reaction:Oxaloacetate + β-NADH → L-Malate + β-NAD

General description

Mitochondrial Isozyme

Malic dehydrogenase is a mitochondrial isozyme and an important catalyst in the tricarboxylic acid cycle.

Physical form

Solution in 50% glycerol containing 0.05 M potassium phosphate buffer, pH 7.5

Unit Definition

One unit will convert 1.0 µmole of oxalacetate and β-NADH to _L-malate and β-NAD per min at pH 7.5 at 25 °C.