Not available outside of the UK & Ireland.

Analysis Note

Protein determined by biuret.

Application

This enzyme is useful for enzymatic determination of triglyceride in serum when coupled with L-α- glycerophosphate oxidase (G3O-301, G3O-311, G3O-321) and glycerol kinase (GYK-301, GYK-311). Usually, the reaction can be completed in 5 minutes at 37^oC by using 2.5 ^oC~3.0 units of the enzyme per test (3.0ml) at pH around 7.0. Lipase from Pseudomonas sp. has been used in a study to assess enzymatic synthesis of biodiesel from palm oil assisted by microwave irradiation.

Biochem/physiol Actions

Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Lipase from Pseudomonas was shown to inhibit monocytes chemotaxis in human peripheral blood.

General description

Lipases are hydrolytic enzymes which break down triacylglycerides into free fatty acids and glycerols.

Packaging

500, 1000 units in poly bottle

Physical form

Lyophilized powder containing Mg⁺², sodium cholate, and bovine serum albumin as stabilizers

Physical properties

Isoelectric point : 5.95 -/+0.05Inhibitors : Hg^++, Ag⁺, ionic detergentsOptimum pH : 7.0 - 9.0 Optimum temperature : 45 - 50^oCpH Stability : pH 7.0 - 9.0 (25^oC, 20hr) Thermal stability : below 55^oC (pH 7.0, 10min)

Unit Definition

One unit will produce 1.0 µmole of glycerol from a triglyceride per min at pH 7.0 at 37 °C in the presence of bovine serum albumin.