Not available outside of the UK & Ireland.

Application

Glutaminase from E.coli has been used:for the determination of glutamine from the cerebral cortex tissue extractfor immobilization on aminopropylsilylated controlled pore glass (CPG) for determination of glutamine in combination with acivicin in in vitro melanoma cells and MCF-7 and OAW-42 cells for cell proliferation and invasiveness assay.

Biochem/physiol Actions

Glutaminase from Escherichia coli along with acivicin, a glutamine analog regulates proliferation and invasiveness of cancer cells. Bacterial glutaminase is implicated in increasing the life span of cancer patients by lowering the tumor burden.

Glutaminase catalyzes the conversion of glutamine to glutamate.

General description

Glutaminase is an amidohydrolase. It has the catalytic domain in the N-terminal and the C-terminal extension possesses STAS domain.

Packaging

10 units in poly bottle

50 units in glass bottle

Physical form

Lyophilized powder containing potassium succinate and EDTA

Unit Definition

One unit will deaminate 1.0 µmole of L-glutamine per min at pH 4.9 at 37 °C.