Not available outside of the UK & Ireland.

Analysis Note

Protein determined by biuret

Application

Glutamic-Pyruvic Transaminase from porcine heart has been used:in the transamination assay with a racemic mixture of synthetic 3-methyl-2-oxoglutarateto remove glutamate from the cell culture medium used for culturing human embryonic kidney (HEK293) cells in the lactate-dehydrogenase (LD)-coupled alanine transaminase (ALT) reaction kinetic studies

Biochem/physiol Actions

Glutamic-pyruvic transaminase (GPT) is located in most cells in the body, but is found in highest concentrations in the liver. It is used as a marker of liver disease due to the release of GPT into the blood following liver cell necrosis.

Glutamic-pyruvic transaminase (GPT) mediates the transfer of the amino group to α-ketoglutarate from l-alanine. It plays a key role in gluconeogenesis and amino acid metabolism. The role of GPT in various kinds of tumors has been reported.

General description

Glutamic-pyruvic transaminase (GPT) is localized majorly in the liver.

Packaging

1000, 2000 units in poly bottle

200 units in glass bottle

Physical form

Lyophilized powder containing citrate and acetate

Unit Definition

One unit will convert 1.0 µmole of α-ketoglutarate to L-glutamate per min at pH 7.6 at 37 °C in the presence of L-alanine.