Not available outside of the UK & Ireland.

Analysis Note

Protein determined by biuret.

Application

Glyoxalase I from Saccharomyces cerevisiae has been used as a standard in Glyoxalase I assay for C.dilatatafemales and brooded encapsulated embryos. It has also been used as a standard for calibration curve generation for quantifying glyoxalase I from brain tissues and cerebral microvessels.

Biochem/physiol Actions

Glyoxalase I is universally expressed and involved in the protection against cellular damage due to cytotoxic metabolites such as advanced glycation end products (AGEs). It is an integral component of the detoxification system, catalyzing the conversion of reactive, acyclic a-oxoaldehydes into the corresponding a-hydroxyacids in a glutathione-dependent manner.

General description

Glyoxalase detoxification system consists of glyoxalase (GLO)-I and GLO-II. GLO-I is a cytosolic, 42 kDa, dimeric Zn²⁺ metalloenzyme.

Packaging

500, 1000, 2500 units in glass bottle

Physical form

Solution in 50% glycerol, 0.4 M (NH₄)₂SO₄ and 0.002 M KH₂PO₄ pH 6.5

Unit Definition

One unit will form 1.0 µmole of S-lactoylglutathione from methylglyoxal and reduced glutathione per min at pH 6.6 at 25 °C.