Not available outside of the UK & Ireland.

Application

Phosphodiesterase (PDE) is an enzyme that is used to breaks phosphodiester bonds. PDE5 is a molecular target for the treatment of erectile dysfunction and pulmonary hypertension. PDE5 is used to find novel PDE5 inhibitors .

Biochem/physiol Actions

PDE5 regulates vascular smooth muscle contraction and is involved in NO-cGMP signaling in platelets to control aggregation. PDE5 may also be involved in the regulation of cGMP signaling in the brain and may modulate pressure-induced cardiac hypertrophy and fibrosis .

Legal Information

TWEEN is a registered trademark of Croda International PLC

Physical form

Supplied as a solution in 20% glycerol containing 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% TWEEN^® 20 and 3 mM DTT

Physical properties

N-terminal GST-tagged 126 kDa full-length protein

Unit Definition

One unit will convert 1.0 picomole of 3′,5′-cGMP to 5′-GMP per minute at pH 7.4 at 37 °C.